Roy Geary, regional director for Northern Europe (including the UK) at Ceva Animal Health, said: “The manufacturing of vaccines is a complex process that involves stringent quality control measures and adherence to regulatory guidelines.
"Unfortunately, the anticipated vaccine batch has failed to meet the quality expected to be suitable for release, which has temporarily affected the ability to meet the demands of the UK sheep market within the main seasonal vaccination period for EAE."
“We anticipate that the issue in supply will be resolved, with some stock potentially available later in the season, however we recognise that for some farmers the supply will arrive too late for them to use.
"We are encouraging vets to explore alternative means of safeguarding the health of their clients’ flocks during this period.
"We recommend implementing robust biosecurity measures and adhering to existing vaccination protocols for other preventable diseases to ensure the overall wellbeing of livestock.
“We will provide vet practices and the wider industry with regular updates as we progress toward a resolution and return to normal supply levels.”
Intervet/Schering-Plough Animal Health has released diagnostic data which the company says indicates that calf scour problems caused by cryptosporidia are on the increase.
A summary of 2010 results from the company's ScourCheck scheme, which identifies infectious scour pathogens in calf faeces, show the protozoan parasite Cryptosporidium parvum being present in 37% of samples.
Intervet/Schering-Plough ruminant veterinary adviser Drew McGurren said: "Ever since we started testing calf faecal samples in the 1990s, rotavirus and cryptosporidia have always been identified as the two main pathogens implicated in infectious scour problems. But the latest data - and feedback from vets in practice - suggest cryptosporidia incidence is on an upward trend.
"Ten years ago our top line reporting used to refer to rotavirus being implicated in around a third of all infectious calf scour problems and cryptosporidia in roughly a quarter. But in 2009, we detected cryptosporidia in 30% of samples. And this year - from the 250 plus faecal samples we have looked at so far - the incidence seems to be even higher."
Drew stressed that scours caused by cryptosporidia are not always confined to housed calves: "Suckler calves between one and two weeks of age can be very susceptible to infection at grass, particularly if conditions underfoot are muddy around ring feeders. Early May has seen quite a lot of rain, and the parasite thrives in damp conditions."
The major source of cryptosporidia is thought to be either adult cows (which act as carriers without showing signs of disease) or infected scouring calves passing the parasite in their faeces. Drew said: "The infectious dose of the organism is very low and if ring feeders are not moved regularly the disease threat that builds up can be very similar to the housed situation. Faecal contamination of feed and water troughs can also be reduced by raising and covering them.
"Cryptosporidiosis in calves is often seen in combination with other diseases, particularly rotavirus. So vaccinating cows against rotavirus with Rotavec-Corona one to three months pre-calving - as well as a sound colostrum feeding regime and maintaining high hygiene standards - can often tip the balance in your favour."
Intervet Schering-Plough says that if cryptosporidia is diagnosed as the problem, parasite replication and excretion can be reduced with Halocur. This is the only product licensed to treat and prevent cryptosporidiosis. Administered orally to calves after feeding (daily for seven days), it can reduce the severity of diarrhoea and prevent the infection spreading to other calves. Treated calves have also been shown to require fewer antibiotic and anti-inflammatory treatments, as well as less rehydration therapy.
Leading industry experts including Faye Murch from Kingsgate Nutrition, Eric Nadeau, Elanco Global Pig Vaccine Advisor, veterinary consultant Daniel Parker and Francesc Molist from Schothorst Feed Research will share their learnings on alternative approaches to managing PWD, including vaccination.
Jack Fellows from Elanco Animal Health says that with 90% of producers using their vet as their first port of call when it comes to PWD1, it is vital they start to encourage producers to consider an alternative prescription to zinc.
He said: “Producers must start to prepare now to avoid being forced to turn to antibiotics when zinc is no longer an option.
"Vaccination will become an increasingly important component of PWD prevention. Amongst other topics on mastering piglet gut health, the seminar aims to support knowledge on the application of in-water vaccines to ensure appropriate and effective use.”
Jack says that while vaccination may appear costly in comparison to zinc oxide, it offers a good return on investment: "Piglets can be vaccinated from 18 days of life, so they are protected from the pathogen at weaning, and we are seeing a growing number of UK herds using vaccines as part of their PWD prevention strategy.
"Farms using the Coliprotec vaccine are no longer using in-water medication to control scours. They’re also noticing that piglets are eating more, and that they’re achieving an extra 2kg of slaughter weight2 while reaching market weights seven days sooner3.”
To register for the event, visit: https://www.myelanco.co.uk/pub/swine-seminar-registration
References
The research will specifically focus on Mycoplasma hyopneumoniae (M.hyop), the causative agent of the disease, and aim to progress vaccine development.
Zoetis has stumped up another £300K in kind to support the research.
M.hyop is present in 80% of UK pig herds, which can result in a 16% reduction of growth and a 14% reduction in feed conversion in pigs.
This therefore, is not only a welfare issue for pigs but can significantly increase production costs for farmers.
Piglets are at particular risk of contracting the disease alongside secondary pathogens during lactation.
There are currently no commercial vaccines available that would prevent initial infection, and while M.hyop is susceptible to a variety of antibiotics, their use needs to be reduced to avoid overuse and the occurrence of multi-drug resistant strains.
The priority for the RVC researchers will therefore be developing new vaccines.
The team will also research the optimisation of protocols to eliminate M.hyop from pig herds in an effort to further minimise potential transmission.
Professor Dirk Werling, Professor of Molecular Immunology at the RVC, said: “Infection of Mycoplasma hyopneumoniae is a really debilitating disease in pigs that causes huge economic losses for farmers.
"I am very pleased that we will be able to continue working with a pharmaceutical partner to develop a new vaccine using state-of the-art technologies.”
Intervet/Schering-Plough Animal Health has launched the Intestinal Health Center for Poultry website (www.ihc-poultry.com), a website to keep producers, veterinarians, nutritionists, academics and students informed about this aspect of poultry production.
The company says it has published a wealth of practical, science-driven information and ideas to help producers manage costly gut diseases more effectively through vaccination, nutrition, sanitation and prudent drug use.
Marcelo Lang, global marketing director for the company's Poultry Business Unit said: "The new website lets you find virtually anything you need, quickly and easily, on specific intestinal health topics and products".
The website also offers a library of past issues of the company's Intestinal Health magazine and its predecessor, CocciForum, as well as three newsletters - Broiler Health, Layer Health and Turkey Health. In addition, the website includes an interactive feature called "Gut Reactions," where visitors can answer a frequently updated questionnaire related to intestinal health, immediately view survey results and then access links with more information on that topic. There is also a product finder to help users find Intervet/Schering-Plough Animal Health intestinal health products available in their market.
Marcelo added: "The Intestinal Health Center for Poultry is another example of Intervet/Schering-Plough Animal Health's commitment to educating the industry about this increasingly important segment of poultry health. We look forward to getting feedback on the site so that we can make it even better."
Julia, who came to the UK in 2016, graduated from the Royal Veterinary College in 2021.
She then completed a master’s degree in animal welfare in Canada, before returning to the UK to join Crowshall in 2024.
Her interest was initially in companion animals, but she decided to focus her career on animal welfare and population medicine.
The judges said: “She has quickly become a dynamic leader in poultry health and welfare, helping farms achieve dramatic reductions in antibiotic use and improved performance.
"Her commitment to evidence-based advice, education and industry advocacy has earned widespread recognition.”
Photo: Julia presented with her award by Shaun Williamson and Emily Wilton from Zoetis.
Guillaume Fournié and Dr Javier Guitian from the Royal Veterinary College - working with colleagues from the London School of Hygiene and Tropical Medicine and Imperial College - have developed a model to understand how live bird markets act as a "hub" of infection for domestic poultry and if rest days, during which such markets are emptied and disinfected, may reduce transmission.
The results are published today in Interface - the Journal of The Royal Society. PhD student Guillaume Fournié, who led the research, said: "Live bird markets can be a reservoir of infection for domestic poultry and may therefore be responsible for sustaining H5N1 HPAI virus circulation.
"Compared to interventions applied in farms - such as stamping out and vaccination - our model shows that frequent rest days are an effective means with which to reduce H5N1 HPAI infection rates. Furthermore, our model predicts that full market closure - as has been implemented in some countries such as Egypt and Vietnam - would only be slightly more effective than rest days to reduce transmission of the disease."
The models used in the study are based on the live bird market chain in Hong Kong and the analysis restricted to chickens. Fournié, who qualified to be a vet in Alfort in his native France, was awarded fully-funded studentships (tuition fees and a living allowance) in 2007 by The Bloomsbury Colleges - a consortium of six colleges of the University of London: Birkbeck, IoE, LSHTM, RVC, School of Oriental and African Studies, and The School of Pharmacy - to conduct the research.
Since December 2003 outbreaks of H5N1 Highly Pathogenic Avian Influenza (HPA1) affecting domestic poultry have been reported in 50 countries across the world. Massive economic losses and the pandemic threat make H5N1 HPAI one of the greatest current public health concerns.
A full copy of the report can be seen in Interface at http://rsif.royalsocietypublishing.org
The company is now asking farm vets to point their clients in the direction of the FarmComm study and encourage them to take part, share their thoughts and help shape the way we all work together in the future.
Mat Yarnall, senior brand manager at Boehringer said: "With routine visits, practice meetings, herd or flock health planning sessions and farming shows and exhibitions all on-hold, how vets work with their clients is subject to change on a scale never seen before.
“The study is striving to understand what methods of working going forward may work, what won’t work and how people want to work.”
The FarmComm study will be being sent out directly to farmers or can be accessed at https://bit.ly/farm-comm.
Matt added: "Following analysis and interpretation, these findings will be shared with vets as we all move together into new and different times."
Novartis Animal Health UK has announced that it is committed to maintaining a reliable supply of Bovidec BVD virus vaccine throughout the coming season.
The announcement comes following concerns surrounding the availability of BVD vaccines. Novartis Brand Manager Eugene Smyth said: "We have been in discussion with the regulatory authorities to try and ensure we can meet the demand over the coming months and we have a large team of territory managers and technical services veterinarians dedicated to supporting our customers through the season".
Bovidec BVD virus vaccine, immunises adult cattle against the BVD Type 1 virus and for active immunisation of calves against BVD Type II virus.
Three new learning aids launched by BVA:
Practical Animal Handling - Online Tutorial The BVA AWF have launched an online tutorial on practical animal handling which has been prepared in conjunction with Newcastle University. The tutorial is primarily aimed at veterinary students and nurses. It features videos of expert veterinary staff demonstrating the correct procedures for handling small animals for clinical examination and medication and its aim is to show that the primary consideration should be for the welfare of the animal. To access the online tutorial visit http://www.bva-awf.org.uk/resources/tutorials/.
Disbudding of Kid Goats The Goat Veterinary Society (GVS) has produced a DVD designed to help veterinary surgeons to disbud goats. Produced in association with the BVA Animal Welfare Foundation the DVD is in memory of the late Mike Teale MRCVS, following a donation from his family. The DVD discusses the potential problems associated with disbudding kid goats and provides guidance on how to avoid them. It shows the best technique for disbudding from start to finish and discusses anatomical considerations, methods of anaesthesia and analgesia and disbudding, with particular attention drawn to the regulatory aspects as they currently apply in the UK. For further information on the GVS DVD, including obtaining a copy, at a cost of £10, please visit http://www.bva-awf.org.uk/headlines/news/Disbudding_of_kid_goats_DVD__2_.pdf.
Bat Care Guidelines - a Guide to Bat Care for RehabilitatorsThe Bat Conservation Trust (BCT) has produced Bat Care Guidelines, in association with the BVA AWF, RSPCA and RCVS Trust, aimed at veterinary professionals, wildlife hospitals and new rehabilitators. The document is split into basic sections including 'health and safety', 'rescue and collection' and 'ailments and treatments' which aim to provide minimum guidelines for bat care. The new initiative is part of an ongoing partnership focusing on the rehabilitation of wild bats in the UK. Visit http://www.bva-awf.org.uk/grants/projects/BCT_workshop_article_08.pdf for further information
Intervet/Schering-Plough Animal Health has produced a new Orf Vaccination Starter Pack to help vets better inform their sheep farmer clients about the disease.
The new campaign Vaccination Starter Pack includes a practical guide to Orf and its control in UK sheep flocks, a DVD profiling vet and farmer experiences of managing the disease, an 'Orf questions answered' handout and a best practice vaccination protocol. Additional practice support materials highlighting the problems caused by Orf and its control with Scabivax Forte are also available.
The Starter Pack and full range of promotional materials are available from local Intervet/Schering-Plough Account Managers or by contacting the Veterinary Support Group on 01908 685685.
As the House of Lords begins its debate on the religious slaughter of farm animals, the RSPCA is calling for all to be pre-stunned.
More than 900 million farm animals are slaughtered for meat in the UK every year. The vast majority are stunned pre-slaughter. However, the law permits the Jewish and Muslim communities to kill animals by religious methods without any form of pre-stunning.
The RSPCA says that while it recognises that religious beliefs and practices should be respected, it also believes that animals should only be slaughtered under the most humane conditions.
RSPCA Dr Julia Wrathall, head of the RSPCA's farm animal science department said: "Farm animals deserve to be treated with care and respect throughout their lives, from the time they are born right up to their final moments in the abattoir.
"We want to see all animals rendered unconscious before slaughter. Until that time we are calling for clear labelling so shoppers are armed with information that can enable them to make an informed choice about whether they buy meat from animals which have not been stunned before slaughter.
"Until there is better, universal labelling, shoppers should look for the blue and white Freedom Food label. Meat produced under this RSPCA assurance scheme comes from animals inspected to the RSPCA's farm animal welfare standards which do not permit slaughter without pre-stunning."
According to the charity, scientific research shows that slaughtering animals without pre-stunning (to render them insensible to pain and distress) can cause unnecessary suffering.
David Bowles, head of public affairs for the RSPCA, who has written a report for the House of Lords debate, added: "We know this is a subject that our supporters feel passionately about. We share their welfare concerns and that is why we have continued to campaign for religious slaughter to be discussed at the highest possible levels."
The RSPCA works with the Food Standards Agency (FSA) to publish the results of the FSA's Animal Welfare Survey undertaken in abattoirs across Great Britain.
The latest survey* results shows:
The RSPCA believes mandatory labelling should be introduced so that all meat that has not been stunned is labelled and says it will press for European rules on this in 2014.
Hysolv Animal Health has announced that it is offering a free swine flu laboratory diagnostic service to veterinary surgeons in the UK and Ireland.
The company will supply kits to veterinary surgeons with which to take samples from pig herds they suspect are infected with swine flu. The samples, nasal swabs or blood, will be sent direct to Germany where vaccine and biological company IDT Biologika is using a special facility to detect which viruses or antibodies against certain serotypes are present.
The virological tests will be conducted by the Institute for Virus Diagnostics, part of the Federal Research Institute for Animal Health. Antibody titres against H1N1, H1N2, H3N2, panH1N1 and panH1N2 will be looked for. Hysolv says these tests, including histopathology, can provide the only conclusive proof of influenza infection.
Results from the test — which would normally cost 230 Euros (about £185) to conduct — will be available within a week. IDT is believed to be the first company offering this type of test.
According to the company, the PCR test — from nasal swabs — is much more precise than previous testing regimes and will identify and type circulating strains as well as emerging strains.
Hysolv says that when swine flu strikes a farm, the signs seen are no longer just typically flu-like but a show whole range including fever, coughing, dyspnoea as well as returns to oestrus and abortions in sows. These may be in combination and concurrent with other diseases which makes accurate diagnosis difficult.
IDT will be offering the newly-branded vaccine Respiporc Flu 3 where the veterinary surgeon feels vaccination in indicated.
Hysolv director Richard Brealey said: “IDT is raising the bar in flu testing and this is a significant advance on what has been done in the past. In 2014 one-third of 1353 nasal swabs sent for testing proved positive for swine flu in Germany. In the UK the samples will help give an insight into the epidemiological status quo of the national pig herd.”
The RCVS has brought out a new publication to help members of the veterinary and veterinary nursing professions understand their legal and professional obligations regarding controlled drugs.
Controlled Drugs Guidance is a consolidation of existing advice and guidance on controlled drugs from a number of organisations including the RCVS itself, the Veterinary Medicines Directorate (VMD), the British Small Animal Veterinary Association (BSAVA) and the Home Office. The guide also gives details of the existing legislation concerning controlled drugs, namely the Misuse of Drugs Act and associated regulations.
The guidance provides advice on a variety of topics to reflect the common queries the RCVS Advice Team receives, such as storage, destruction and disposal; keys and keyholders for controlled drugs cabinets; controlled drugs in vehicles; and veterinary nurses administering controlled drugs.
The publication also deals with specific controlled drugs such as ketamine which, on 30 November 2015, was rescheduled to a Schedule 2 controlled drug, meaning that it is now subject to the same strict storage, prescription, dispending, destruction and record-keeping requirements as other medicines in this Schedule.
Laura McClintock, Standards and Advisory Manager at the RCVS, said: “We hope that this publication will help members of the profession navigate the often complex legislative and professional requirements regarding controlled drugs, as well as imparting best practice advice on how they should be stored and disposed and so on.
"This publication has also been endorsed by our colleagues in the Home Office, VMD and BSAVA and, because this is an area that is subject to regular change, we will make sure to keep it up-to-date as and when the regulations change."
The publication is available to download from the RCVS website at www.rcvs.org.uk/publications
Zoetis has released new surveillance results which show that Mycoplasma bovis is becoming an increasingly significant pneumonia pathogen in cattle1.
In more than 2,500 samples submitted by vets for analysis during 2014, 45% were positive, compared with 41% in 2013.
According to Dr Tim Potter MRCVS from Westpoint Veterinary Group, Mycoplasma bovis has emerged as an important pathogen of calves worldwide2. He said: "It is associated with a number of clinical presentations including respiratory disease, otitis media and arthritis.
"The role of Mycoplasma bovis in pneumonia is well established and there are a number of reports in the literature of respiratory disease outbreaks where it was the predominant bacterium isolated. However, as with most causes of pneumonia, colonisation by Mycoplasma bovis alone is not always sufficient to cause disease and it may present in combination with other respiratory pathogens."
For other pathogens, the surveillance found 83% of samples positive for Para-influenza 3 virus (Pi3v), 76% Bovine Respiratory Syncytial virus (BRSv), 32% IBR, and 25% for BVD. Apart from BVD, which saw a three-point reduction from 28% the previous year - these show little change from 2013.
According to Zoetis vet Carolyn Hogan, in pneumonia outbreaks involving Mycoplasma bovis, many of the clinical signs are similar to those caused by other pneumonia pathogens: coughing, raised temperature, rapid breathing and lethargy.
She said: "This can make it difficult for farmers and vets alike to identify Mycoplasma bovis as a possible cause of disease based on clinical signs alone, unless calves are also showing some of the other distinguishing signs associated with Mycoplasma infection.
"These include ear infections, head shaking or tilting, and arthritis. Poor response to treatment, with resulting chronic respiratory cases, may also be suggestive of Mycoplasma infection. For a definitive diagnosis, of course, analysis of blood or respiratory tract mucous is required."
Whilst vaccination protection is available against the viruses (BRSv, Pi3v, BVD and IBR) and Mannheimia (Pasteurella) haemolytica, Carolyn points out that there is no ‘off the shelf’ vaccine available for Mycoplasma bovis. She adds that treatment can be challenging due to the insidious nature of disease, and lack of effectiveness of some antibiotics.
Once Mycoplasma bovis is present on a farm, Carolyn cautions that eradication can be very difficult. "It can be transmitted in milk from carrier cows, and in saliva among calves, particularly when multiple animals suckle the same teat feeders.
"Minimising this pathogen's impact on health and productivity relies on reducing its exposure to susceptible animals and maximising their resilience against it. For a comprehensive programme of control measures, farmers should consult their vet."
Carolyn suggests that such a programme is likely to include: testing the farm's Mycoplasma bovis status; effective biosecurity; not feeding infected milk; ample ventilation for adults and youngstock alike; rapid spotting of sick animals, isolation and vet-prescribed treatment; vigilance for poor response to treatment and development of chronic cases; vaccination against main viral pneumonia pathogens; and low stress through good stockmanship.
Photo: By Y tambe (Y tambe's file) [GFDL, CC-BY-SA-3.0 or CC BY-SA 2.5-2.0-1.0], via Wikimedia Commons
Scientists at the Royal Veterinary College, contributing to a programme to tackle some of the most harmful and widespread endemic diseases that commonly affect farmed animals in the UK, have developed a severity estimation calculator for post-weaning multi-systemic wasting syndrome (PMWS).
Post-weaning multi-systemic wasting syndrome (PMWS) is a common disease of young pigs in the U.K. It is extremely debilitating, causes considerable suffering and poor welfare, and has a high mortality of up to 30%. It is estimated that the disease costs the UK farming industry £30m per year.
The £2.4m project - headed by Professor Dirk Werling from the Royal Veterinary College and funded by the Biotechnology and Biological Sciences Research Council (BBSRC) through its "Combating Endemic Diseases For Farm-Animal Sustainability" programme (CEDFAS), the British Pig Executive (BPEX), and two industrial partners, Pfizer Ltd (UK) and BioBest - aims to identify why PMWS occurs and to develop new methods of controlling the disease.
One key-component of the project is the direct involvement of British pig farmers, thereby guaranteeing that pigs in Britain will be the first to benefit from this research, while collaboration with a major international pharmaceutical company will ensure that new veterinary products will be made available to pig farmers worldwide.
Now, two members of the team, Dr. Barbara Wieland and Pablo Alarcon-Lopez, a second year PhD student, have developed a novel approach to quantify severity of PMWS based on morbidity and mortality data and presence of porcine circovirus type 2 (PCV2). They applied factorial analyses, using data collected from 147 pig farms in England to identify variables contributing to variation in PMWS severity in a biologically meaningful way.
Together with other known variables linked to PMWS, the resulting factors were used to derive an algorithm for PMWS severity. The derived algorithm was used to develop a PMWS severity calculator, which now can be used by farmers directly to assess the PMWS severity on their own farm (http://www.bpex.org.uk/articles/300874).
The levels of PMWS severities identified on farm level can now be used to identify risk factors related to the different severity-categories, to assess the efficacy of PCV2 vaccination and to investigate the economic impact of potential control measures on a farm.
The Veterinary Laboratories Agency has worked in collaboration with the Institute of Animal Health (IAH) at Pirbright to introduce the Polymerase Chain Reaction (PCR) test for Bluetongue virus.
This test is now available via veterinary surgeons for pre-movement (UK) and export testing purposes only.
As of this date, PCR testing for these purposes will no longer be available from IAH, Pirbright.
For further information see www.vla.gov.uk
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Following stock challenges earlier in the year, the company is now urging vets to encourage farmers to vaccinate their flocks to prevent abortions during lambing.
Roy Geary, regional director for Northern Europe at Ceva Animal Health, said: “While we realise that the temporary delay of Cevac Chlamydia has been inconvenient to our valued sheep farmers we would like to thank all our customers for their patience and support during this challenging time.
"The vaccine supply is now available in the UK for the latter end of the season.
"Farmers tupping later in October should therefore be encouraged to vaccinate their flocks to protect their ewes from this devastating disease.”
www.enzooticabortion.co.uk
Virbac Animal Health has launched a special scheme to encourage farmers to have milk samples analysed in line with the industry-wide campaign to promote the responsible use of antimicrobials.
Under the terms of the scheme, veterinary surgeons purchasing Virbac's Cephaguard DC therapy will receive vouchers to distribute to their farmer clients. These vouchers can be redeemed for milk bacteriology to determine the pathogens causing mastitis and so ensure treatment is more targeted and effective.
Brigitte Goasduf MRCVS, Ruminants Marketing Manager at Virbac, said: "Mastitis is a major concern for dairy farmers with reported numbers of cases ranging from 26-50 for every 100 cows.
"Experts agree that individual SCC monitoring and the accurate diagnosis of pathogens causing infection are key to treating mastitis effectively. Unfortunately many farmers are often deterred by the cost.
"Our scheme gives our veterinary customers the opportunity to foster good practice by encouraging farmer clients to submit milk samples for investigation at no cost to them. This will then enable them to treat the bacteria causing the infection in line with industry guidelines for the use of antimicrobials.
"Over time, we believe farmers will come to realise that adopting this more targeted approach is not only more cost-effective but will also preserve their ability to treat their herd, both now and in the future."
For further information on the scheme, contact your Virbac territory manager or call Virbac on 01359 243243.
US-based Life Technologies has launched a new PCR-based test system which, according to the company, allows large numbers of pigs to be screened more quickly and cost-effectively for a range of common pathogens, such as PRRSV, SIV and PCV2.
The system uses samples of oral fluids which can be obtained by leaving a cotton rope in each pen for pigs to chew on. After 20 minutes or so, the rope can be retrieved and the saliva and gingival crevicular fluid squeezed into a sample bag and represents a pooled sample from the group.
Studies have demonstrated that oral fluid samples collected in this way can form the basis of a quick and cost-effective method for screening a range of common viral pathogens. The samples were tested using a commercially available sample preparation and real-time PCR test system which can isolate and identify viral nucleic acid in a matter of hours.
For PRRS, pooled samples of oral fluids were collected from groups of experimentally infected pigs using the rope technique, along with serum samples from each individual pig on the same days. Both serum samples and oral fluids were processed using the same Applied Biosystems preparation system and real- time PCR test, both of which were supplied by Life Technologies. The results showed that PRRSV nucleic acid was detectable in both serum and oral fluid samples from the day of infection through to 40 days after infection.
For PCV2, 24 pigs that were free of PRRSV and SIV were divided into 4 pens in separate rooms, and challenged with two different virus strains (PCV2a and PVC2b) at different times. One pen acted as a (non-challenged) control. Oral fluids were collected regularly up to 140 days after initial challenge and tested using real-time PCR. High titres of PCV2 were detected from day 12 to day 28 post infection and virus was detectable throughout the entire testing period (days 2 to 98).
For SIV, a total of 180 spiked oral fluid samples were tested using real-time PCR, and subtyping reagents were also used to identify haemagglutinin and neuraminidase subtypes. The results showed that SIV nucleic acid was detectable in oral fluid samples spiked with high, medium and low copy numbers of SIV, and all positive samples could be successfully sub-typed.
Christina Boss, European Professional Service Veterinarian for Life Technologies said: "Collecting samples in this way is far less invasive for the pigs and so avoids unnecessary stress. And because all of the pigs will chew on the rope, it provides a very broad sample from the group, which is the key to assessing overall herd health. In addition, if the pooled sample provides a positive result, then the animals in that pen can be tested individually to identify those that are infected.
"These results show that the simplicity of oral fluid sampling, combined with the speed and sensitivity of a PCR-based system, provides a practical and cost- effective way of monitoring large numbers of pigs for common virus pathogens.
"The use of a semi-automated, PCR-based diagnostic system means that nucleic acid purification can be achieved in just 25 minutes and results from the real-time PCR available in 90 minutes. The molecular test is very specific and reliable, so the veterinarian can initiate individual testing as quickly as possible and make confident recommendations to the producer."
According to Life Technologies, screening for PRRS using samples of oral fluids has been gaining popularity over recent years because large numbers of pigs can be tested without increased cost or labour. The new research is due to be presented as a poster at the ESPHM meeting in Bruges in April.
The collaborative study, which was sponsored by Norbrook, involved each practice blood testing a cohort of six lambs on 25 individual farms from 16 weeks of age, to look for evidence of antibodies to liver fluke.
Only 11 farms had positive results in the period from June, up to December, with most only showing one or two lambs out of the six sero-converting later in the year. Even farms located reasonably close by showed positive cases months apart.
Mark Thompson of Craven Farm Vets in Yorkshire said, “I did not expect to see positive results as late in the year. I expected a positive result earlier (September/October) as the farm is a very wet farm with a long-standing issue with fluke. Also, the weather in the area has been ideal for the intermediate host and fluke development on the pasture.”
Emily Baxter of Drove Farm Vets in Wiltshire said she thinks there is scope to use the results to improve the timing of dosing with flukicides and more responsible use: “We will use the results from this year’s testing to re-evaluate the farm’s fluke control protocol which will help reduce the level of use of flukicides earlier in the season for years with similar weather patterns and assist in planning of strategic grazing.
"We’re looking forward to continuing our work this year, no two years are ever the same, that’s why monitoring is so important. It will allow both ourselves and our clients to build up a picture over time of what’s happening not only on their individual farms, but potentially down to field level! This will allow us to provide more conclusive recommendations. For now, it’s clear that testing has great promise as a means to provide tailored advice to farms on how to control fluke and use the flukicides available to us responsibly.”
Emily says it’s important that farmers do not stop using flukicides based on the results of this study nor assume that there is less risk: “We all know how significant the losses from acute and chronic fascioliasis can be. We therefore encourage farmers to talk to their vet before they change their flukicide treatment protocols and discuss the possibility of using serology as an adjunct to determine how and when they treat.”
Veterinary professionals can find out more about the fluke sentinel project at https://xlvets-farm.co.uk/fluke-sentinel or the XLVets community at https://www.xlvets.co.uk/the-boss/.
The new award, sponsored by Krka UK, will recognise an exceptional farm vet who has been working in the dairy industry for three to five years and who has contributed significantly to the health and wellbeing of herds under their care.
Sarah Tomlinson, veterinary surgeon at Westpoint Farm Vets in Derbyshire, Technical Director of the TB Advisory Service (TBAS) and recipient of the 2020 Dairy Vet of the Year, said: “In recognising achievement among more recently qualified vets with this new award, Krka is giving the boost every young vet needs to shake off the ‘new grad’ title and instead become an ‘high performing’ vet, judged by their industry peers to be one of the best. Many vets can suffer with imposter syndrome so it is important that we accept positive acknowledgement of our work and our everyday achievements so that we can be rightly proud of what we do.”
More information about the CREAM Awards and the nomination process can be found here: https://www.britishdairying.co.uk/cream-awards-2021/