To raise the funds, CVS put customer and staff collection boxes in its small animal first opinion practices and referral hospitals.
Staff also held a number of fundraising events – including sponsored walks and dog shows, as well as hosting Pet Blood Bank donation days.
The money raised from the partnership will help go towards funding Pet Blood Bank’s work.
Last year the charity sent over 5,000 units of blood to vets across the UK.
Pet Blood Bank provides training for vets and nurses, and donations also help the charity to keep the cost of blood products as low as possible, and provide them free to veterinary charities.
Deputy Chief Executive of CVS Group, Ben Jacklin, said: “Pet Blood Bank is a vital charity doing crucial work within our profession.
"There is no doubt that the level of funds raised is down to the tireless commitment shown by our colleagues up and down the country.
"I’d also like to recognise the role our clients have also made - as without them we would never have reached such a great total.”
Managing Director of Pet Blood Bank, Katrina Wilkinson said: “This generous donation from CVS Group has been incredible to receive.
"We are a small charity so need all the support we can get, and CVS has truly delivered."
CVS has recently announced its next charity partner as Guide Dogs for the Blind.
Pet Blood Bank UK (PBBuk) launched its new mobile blood collection unit, donated by Royal Canin, at the University of Nottingham's School of Veterinary Medicine and Science on Wednesday, simultaneously collecting enough blood to save the lives of up to 32 dogs.
The blood collection session offered third-year university students an opportunity to learn about transfusion medicine and to practice their clinical skills on real life subjects, as they performed the pre-donation checks and tests under the supervision of Pet Blood Bank UK veterinary advisers.
Vanessa Ashall, Pet Blood Bank UK welfare officer and veterinary surgeon, said: "It is great for students to be able to see these animals. Transfusion medicine is a new science and I certainly wasn't taught about it as a veterinary student. Collaboration with veterinary schools is such a positive step, as we are able to educate the vets of the future on the benefits of transfusion medicine and blood typing, whilst giving them some real hands-on experience."
Veterinary support manager for Royal Canin, Chris Geddes, said: "We are really pleased to be able to assist Pet Blood Bank UK become more mobile and reach more donors. The charity's work is so important to the emergency and critical care of patients, as shown by the tremendous gratitude of the owners of blood recipients."
Golden retriever Beau was one such recipient who had received a transfusion last month after suffering a haemorrhage after surgery for gastric dilatation and volvulus. His owners, Janet and David Liggins, attended the event with a fully-recovered Beau and their other golden retriever, Lotti who gave blood.
Mrs Liggins said: "We can't speak highly enough of all the vets and vet nurses that cared for him - they did a tremendous job looking after Beau, and us! The Pet Blood Bank provided the blood that saved Beau's life. We are really pleased that Lotti was able to give blood. She was a star - the perfect donor!"
Veterinary practices wanting to encourage their clients to put dogs forward as donors, or which would like to support the work of Pet Blood Bank UK, should visit http://www.petbloodbankuk.org/ or contact Pet Blood Bank UK on 01509 232 222 or 0844 800 9925 (5p/min). Blood donors are needed for a second blood collection session at the Nottingham School of Veterinary Medicine and Science, Sutton Bonington campus, on Saturday, May 23: 10-4pm.