https://www.vetsurgeon.org/f/clinical-questions/11787/genetics-of-mast-cell-tumours Hi, I have a client with a 2 year old, labrador retriever, which had a mast cell tumour removed from the right thorax in March. The owners would like to breed from this bitch, and have asked me my advice. The histology results confirmed an intermiediateen-USTelligent Community 10https://www.vetsurgeon.org/thread/64859?ContentTypeID=1Sat, 02 Jun 2012 10:18:54 GMT146601cc-3922-4be7-9974-7e1d4e45a66b:7aaed7a2-8ed3-4de4-92b4-3c1c2e281e33Richard Fox<p>I am afraid no one know yet&nbsp; - unless full sequencing of cKIT (gene encoding for KIT/CD117 protein receptor) is performed in large numbers of labs then we wont know also many animals have and require multiple mutations in their genes/Mast cells for neoplasia to evolve - not just as simple a having cKIT mutation alone? To my understanding most dogs do not have cKIT mutations - most low and intermediate give a membranous KIT immunostaining pattern and thus putatively non-mutated - mutated cKIT is general seen in upper grade 2 and grade 3 tumours which are less frequent (depending on how brave the pathologist grading are ;) ) Unless you do DNA amp/PCR testing you don&#39;t know if cKIT is mutated but the localisation gives you a reasonable correlation :)</p><div style="clear:both;"></div>